Cell Wall Alterations Associated with the Hyperproduction of Extracellular Enzymes in Neurospora crassa
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چکیده
منابع مشابه
Structure and composition of the cell wall of Neurospora crassa.
The structure and composition of the cell walls of hyphae of Neurospora crassa were investigated by electron microscopy, chemical analysis, and X-ray diffraction both before and after progressive enzymatic degradation by snail gut enzymes, chitinase, and trypsin. The wall consists of two phases: randomly disposed skeletal microfibrils of chitin only and an amorphous matrix which contains both b...
متن کاملMajor extracellular protease of Neurospora crassa.
The inducible extracellular alkaline protease of Neurospora crassa was demonstrated to be a glycoprotein containing D-galactose residues by use of the enzyme-lectin conjugate horseradish peroxidase-Ricinus communis-agglutinin-120. The carbohydrate moiety of the protease appears to be a poor antigen since an antiserum made to the native enzyme recognizes epitopes determined only by the polypepti...
متن کاملCell Fusion in Neurospora crassa
The primary research aims of this thesis were the identification of novel cell fusion mutants of Neurospora crassa and the subsequent functional characterization of selected candidate proteins during conidial anastomosis tube (CAT)-mediated cell fusion by means of genetic, molecular, biochemical and live-cell imaging analysis. Chapter 1 provides a general introduction of the model organism and ...
متن کاملMOLECULAR ANALYSIS OF THE SULFUR REGULATORY CIRCUIT OF NEUROSPORA CRASSA
The sulfur regulatory circuit of the filamentous fungus, Neurospora crassa, consists of a set of unlinked structural genes which encode sulfur catabolic and two major regulatory genes which govern their expression. The cys-3 regulatory gene encode a transacting regulatory protein which activates the expression of cys-14 and ars, whereas the other regulatory genes Scon-l and Scon-2 appear to...
متن کاملThe peptides of the hyphal wall of Neurospora crassa.
The peptides of the hyphal wall of Neurospora crassa have been studied to determine their physical characteristics. We have removed these peptides from the wall by treatment with 05 M-NaOH and shown by DEAE cellulose chromatography that there are five major fractions present. Amino acid analysis showed distinct differences in primary structure between these fractions but similarities in the pro...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1972
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.111.2.443-446.1972